Jupita Automatic Fluorescence Cell Analyzer

Jupita is a full-automatic multi-channel cell fluorescence analyzer introduced by Newton Optics. It is based on image detection and combines multi-fluorescence channels to collect cell information in the image for quantitative analysis of cell samples. Jupita combines cell image analysis and counting with fluorescence analysis to provide both bright-field cell counting statistics and analysis results for up to 12 combinations of fluorescence channels.

Jupita is a full-automatic multi-channel cell fluorescence analyzer introduced by Newton Optics. It is based on image detection and combines multi-fluorescence channels to collect cell information in the image for quantitative analysis of cell samples. Jupita combines cell image analysis and counting with fluorescence analysis to provide both bright-field cell counting statistics and analysis results for up to 12 combinations of fluorescence channels.

Patented fully automatic/autofocus cell fluorescence analysis system
Jupita adopts Newton optical self-developed automatic cell fluorescence analysis control scheme, and cooperates with stepping motor and joystick to control sample entry, movement and precise positioning, thus realizing multi-channel and multi-field sampling. Jupita can fully automatic focus processing of samples to ensure clear images and accurate results. The design of double laser beam excitation light source and filter turntable greatly improves the stability and operability of the system.
 

Reliable, accurate and fast image processing system
Jupita fluorescence analyzer uses industrial-grade high-pixel CMOS, LED excitation light source and filter. In the counting process, each sample is sampled in three fields of view, and the image information of the sample bright field and selected fluorescence channel is collected at the same time. Larger sample collection ensures the accuracy of the count, and it is verified that the fitting R2 value of the count result is greater than 0.999 *. With the integrated control system and efficient algorithm, Jupita cell analyzer can complete the fluorescence analysis of a single field of view in 10 seconds, and the fluorescence analysis results of 6 samples and 18 sampling fields can be obtained in 5 minutes.
 

* Results from cell count tests on RAW264.7,Siha at gradient concentrations, respectively
* Results from a single six-sample fully automated test of suspension culture cells at a concentration of 1 × 106
 

Fully automatic multi-fluorescence synchronous detection, a variety of applications at a touch
Jupita can provide up to 4 excitation lights and 2 filters to meet the needs of various cell applications. Jupita has a built-in full-automatic optical path switching system. A single experiment can detect the results of 2 channels at the same time. The software presets a variety of commonly used dyes/fluorescent proteins and corresponding spectral combinations for selection. You only need to set up the required experimental scheme and leave the rest to Jupita.
 

Humanized, multi-functional interactive interface
Jupita fluorescence analyzer with computer *. Users can freely set the analysis mode and specific analysis settings (currently, brightfield/trypan blue/AO/PI/AOPI/GFP counting mode can be selected, samples can be specified, and dilution concentration can be adjusted). The software provides detailed data such as analysis result chart of each channel, concentration, activity rate, diameter distribution, and image merging function of different fluorescence channels for users to view and export data in various formats to meet different user needs. In addition, the software provides historical data traceability and user rights management * to meet FDA 21 CFR Part 11 regulatory requirements.
* Recommended configuration: i5 CPU, 4G memory, 64GB hard disk, 1024*768 resolution, Windows10 system or above
* Hierarchical permissions function, only administrator users can operate user rights management, including new users, delete users


 

Activity Count-AO/PI Double Fluorescence Staining
 

EasyFive cell counting plate
Patented design six cell counting plate *, high throughput design, single counting plate can count 6 samples. Disposable consumables design, no need to clean, save time, eliminate cross-contamination, and reduce the risk of biological hazards to laboratory personnel. 100 μm(14μL)/200 μm(25μL) specifications are optional *,100μm counting plate is suitable for high concentration or small diameter cells, and 200μm counting plate is suitable for various common cells, which can be matched according to sample conditions.
 

* A cell counting special sample pool and preparation method. ZL 201910136771.6 (Submitted)
* Two kinds of counting plate specifications are available, the sampling volume of 100μm counting plate is 15μL, and the sampling volume of 200μm counting plate is 25μL
 

Cell Transfection-GFP Fluorescence Detection

* Experimental images from the Newton Institute of Optics, both activity counts and cell transfection using Hela cells